Quick Answer: What Happens To A Primary Transcript In Eukaryotic Cells Before Translation Can Occur?

What is a primary transcript a strand of?

A primary transcript is the single-stranded ribonucleic acid (RNA) product synthesized by transcription of DNA, and processed to yield various mature RNA products such as mRNAs, tRNAs, and rRNAs.

The primary transcripts designated to be mRNAs are modified in preparation for translation..

What is the difference between protein synthesis in prokaryotes and eukaryotes?

In prokaryotes, mRNA molecules are polycistronic containing the coding sequence of several genes of a particular metabolic pathway. In eukaryotes, protein synthesis occurs in the cytoplasm. In prokaryotes, protein synthesis begins even before the transcription of mRNA molecule is completed.

How is the primary transcript processed after transcription in eukaryotic cells?

RNA polymerase II of eukaryotes transcribes the primary transcript, a transcript destined to be processed into mRNA, from the antisense DNA template in the 5′ to 3′ direction, and this newly synthesized primary transcript is complementary to the antisense strand of DNA.

What is the difference between prokaryotic and eukaryotic cells in transcription and translation?

In a prokaryotic cell, transcription and translation are coupled; that is, translation begins while the mRNA is still being synthesized. In a eukaryotic cell, transcription occurs in the nucleus, and translation occurs in the cytoplasm.

Is translation the same in prokaryotes and eukaryotes?

Translation is a universal process occurs in both prokaryotes and eukaryotes. The fundamental process of translation is same in prokaryotes and eukaryotes. Members of both groups uses information present in mRNA, which is came from the DNA by transcription, to synthesize proteins with ribosome as the machinery.

Why is attenuation not found in eukaryotes?

There is no coupling of transcription and translation in eukaryotic cells as in bacterial cells. Consequently, ribosome movement and arrest cannot modulate attenuation.

How come prokaryotes can both transcribe and translate a gene at the same time but eukaryotes Cannot?

Prokaryotic transcription occurs in the cytoplasm alongside translation. Prokaryotic transcription and translation can occur simultaneously. This is impossible in eukaryotes, where transcription occurs in a membrane-bound nucleus while translation occurs outside the nucleus in the cytoplasm.

What are the major differences in transcription between prokaryotes and eukaryotes?

There is no such structure seen in prokaryotes. Another main difference between the two is that transcription and translation occurs simultaneously in prokaryotes and in eukaryotes the RNA is first transcribed in the nucleus and then translated in the cytoplasm.

Why is RNA processing important for eukaryotes?

Eukaryotic mRNAs must undergo several processing steps before they can be transferred from the nucleus to the cytoplasm and translated into a protein. … The mRNA transcript is coated in RNA-stabilizing proteins to prevent it from degrading while it is processed and exported out of the nucleus.

What are the major differences between translation in bacteria and translation in eukaryotes?

Translation has three main stages: initiation, elongation, and termination. These differ slightly in prokaryotic and eukaryotic organisms: in prokaryotes, translation occurs in the cytoplasm, while in eukaryotes, translation takes place in the endoplasmic reticulum.

What steps are needed to produce a mature mRNA?

In the nucleus, a pre-mRNA is produced through transcription of a region of DNA from a linear chromosome. This transcript must undergo processing (splicing and addition of 5′ cap and poly-A tail) while it is still in the nucleus in order to become a mature mRNA.

Does translation occur in eukaryotic cells?

In a eukaryotic cell, transcription occurs in the nucleus, and translation occurs in the cytoplasm.

Where do transcription and translation occur in eukaryotic cells?

The eukaryotic nucleus therefore provides a distinct compartment within the cell, allowing transcription and splicing to proceed prior to the beginning of translation. Thus, in eukaryotes, while transcription occurs in the nucleus, translation occurs in the cytoplasm.

What happens to mRNA during processing?

RNA splicing. The third big RNA processing event that happens in your cells is RNA splicing. … During splicing, the introns are revmoved from the pre-mRNA, and the exons are stuck together to form a mature mRNA that does not contain the intron sequences.

What happens to the mRNA after processing?

The process of removing the introns and rejoining the coding sections or exons, of the mRNA , is called splicing. Once the mRNA has been capped, spliced and had a polyA tail added, it is sent from the nucleus into the cytoplasm for translation.

Where does the process of translation occur in eukaryotic cells?

The eukaryotic nucleus therefore provides a distinct compartment within the cell, allowing transcription and splicing to proceed prior to the beginning of translation. Thus, in eukaryotes, while transcription occurs in the nucleus, translation occurs in the cytoplasm.

What are 4 differences between prokaryotic and eukaryotic cells?

Following are the substantial difference between Prokaryotic Cells and Eukaryotic Cell: … Organelles like mitochondria, ribosomes, Golgi body, endoplasmic reticulum, cell wall, chloroplast, etc. are absent in prokaryotic cells, while these organelles are found in eukaryotic organisms.

What are the steps of translation?

Translation of an mRNA molecule by the ribosome occurs in three stages: initiation, elongation, and termination. During initiation, the small ribosomal subunit binds to the start of the mRNA sequence.

Why can’t translation begin before transcription in eukaryotes?

Prokaryotic transcription occurs in the cytoplasm alongside translation. Prokaryotic transcription and translation can occur simultaneously. This is impossible in eukaryotes, where transcription occurs in a membrane-bound nucleus while translation occurs outside the nucleus in the cytoplasm.

What are the three steps of pre mRNA processing in eukaryotic cells?

The three most important steps of pre-mRNA processing are the addition of stabilizing and signaling factors at the 5′ and 3′ ends of the molecule, and the removal of the introns ((Figure)). In rare cases, the mRNA transcript can be “edited” after it is transcribed.

What are the 3 major steps involved in mRNA processing?

what are the three major steps of mRNA processing? Splicing, adding of the cap and tail, and the exit of the mRNA from the nucleus.